Abstract
Chromatographic behavior of aromatic amines on reversed-phase HPLC was investigated and the obtained HPLC conditions were applied to the analysis of carcinogenic isomers in commercial aromatic amines. In this study, it was found that most of aromatic amine isomers were well separated from each other by using methanol-McIlvaine buffer solution as a mobile phase. Analytical methods for carcinogenic isomers in three commercial aromatic amines, i.e., 2-naphthylamine in 1-naphthylamine, 2-anthracenamine in 1-anthracenamine, and 2-aminoanthraquinone in 1-aminoanthraquinone, were developed by applying the conditions obtained in this investigation. All of carcinogenic isomers were eluted faster than primary components of the commercial aromatic amines labeled, so that these isomers could be easily determined without interference.
