1989 Volume 38 Issue 11 Pages 627-631
High sensitive spectrophotometric determinations of serum phospholipids were conducted. The levels of serum phospholipids reflect liver functions, so thier determinations are clinically important. Phospholipids are hydrolyzed by phospholipase C and alkakine phosphatase with the release of phosphate. The resulting phosphate associated with Mo-MG reagent (mixed solution of ammonium molybdate and Malachite Green) to form molybdophosphate-Malachite Green aggregates. The aggregates were selectively collected on a nitro cellulose membrane filter (pore size:3.0 μm) and dissolved in methylcellosolve along with the membrane filter. Absorbance (λ: 627 nm) was proportional to the amount of phospholipids. Molar absorptivity of the aggregates in methylcellosolve (ε=2.8 × 105 M-1 cm-1) was large enough to minimize the serum sample for determination. Ascorbic acid, bilirubin, Na+, K+, Ca2+ and Cl- in serum did not affect the results obtained by this method.