1989 Volume 38 Issue 11 Pages 650-652
HPLC using a chiral mobile phase containing either D-or L-phenylalanine-copper(II) complex was evaluated for purity testing of (S)-(-)-ofloxacin (OFLX). As a result, D-phenylalanine was found to be a suitable chiral additive for optical purity testing of (S)-(-)-OFLX. The detection accuracies obtained with a UV detector at 294 nm and a fluorescence detector at Ex. 330, Em. 505 nm, were compared. It was found that the fluorescence detector could detect with greater stability much less (R)- (+)-OFLX in the presence of (S)-(-)-OFLX than the UV detector. (S)- and (R)-OFLXs in spiked serum were determined simultaneously by connecting a UV detector and a fluorescence detector in series.
