Comparison of serum theophylline level determinations by Vision® Analyzer and fluorescence polarization immunoassay

Abstract

We have compared the serum theophylline levels determined by Vision® Analyzer and fluorescence polarization immunoassay (FPIA). Eighty-five blood samples were collected and the serum was analyzed for theophylline using both methods. The equation for the regression line is Visio®=0.88 × FPIA+1.21, r=0.97. Total bilirubin at 17.2 mg/dl and hemoglobin at 230 mg/dl caused interference that was detected by the Vision® Analyzer. Serum theophylline determined on Vision® is precise and agrees with an established procedure.