1991 Volume 40 Issue 8 Pages 383-387
A dialysis membrane-covered, graphite paste electrode was prepared and tested as a detecting electrode for uric acid in serum. The current magnitude of uric acid on the linear sweep voltammograms was proportional to the concentration of uric acid ranging from 1 μM to 200 μM. Protein, which fouls the surface of bare electrode and worsens the property of the electrode, did not affect the anodic current of uric acid at the membrane-covered electrode. Oxidizable compounds in serum except ascorbic acid did not interfere with the current of uric acid measured at 0.5-0.6 V in the acidic solutions by this electrode. The magnitude of the anodic current of a serum solution measured in pH 3-4 could be corrected for the effect of ascorbic acid to obtain the current due to uric acid. Values of uric acid content in serum measured by this method agreed with those by a photometric uricase-peroxidase procedure.
