HPLC determination of inositol in multivitamin preparation containing large amounts of sugars

Abstract

A simple high-performance liquid chromatographic method was developed for the determination of inositol in multivitamin preparations containing large amounts of sugars. The sample multivitamin preparation was separated on an acrylic amide column with acetone-water (7 : 3) as the mobile phase and an RI detector. The quantitations were achieved by the peak area ratio method using α-cyclodextrin as an internal standard. Inositol in the multivitamin preparations containing large amounts of sugars was determined successfully by the present method without any pretreatment.