Enantiomer separation of small amounts of amino acids and hydroxy acids by HPLC using chiral stationary phases and achiral derivatizing reagents

Abstract

The enantiomer separation of small amounts of amino acids and hydroxy acids by HPLC using chiral stationary phases and achiral derivatizing reagents was examined. Racemic dabsyl amino acids and pyrene-l-carbonyl hydroxy acids were well resolved on chiral stationary phases [VI] and [V], and excellent values of detection limits were obtained. The joint use of chiral stationary phases and achiral derivatizing reagents was found to be very effective for the separation and determination of small amounts of amino acid and hydroxy acid enantiomers by HPLC.