Simultaneous determination of pesticides including triclopyr and triclopyr butoxyethyl in drain-water samples from golf courses by solid-phase extraction/HPLC

Abstract

We have developed a method for the simultaneous determination of 16 kinds of pesticides, including triclopyr and triclopyr butoxyethyl, in drain-water samples from golf courses by solid-phase extraction/HPLC. Using a polymethacrylate column (Golf-Pak HR 150 × 46 mm i.d.), 16 kinds of pesticides could be separated by changing from 50 mM phosphate buffer (pH 3.3)/acetonitrile = 70/30 → 50/50 (0 → 8 min) -42/58 (8 → 15 min) -42/58 → 30/70 (15 → 25 min) as the mobile phase by a gradient program. The time required for the analysis was about 30 minutes, including the time for initializing. The linearities of the responses (peak area) versus the concentration of 16 kinds of pesticides were examined over the range from 0.060.4 mg/l to 10 mg/l. The mean recovery of 16 kinds of pesticides from pure water at a concentration of 0.008 mg/l was 75 107%, and the mean relative standard deviation was 1.6 5.7%. Using two solid-phase extraction cartridges connected in series, the mean recovery for 16 kinds of pesticides from river-water samples at a concentration of 0.008 mg/l was 73119%, and the mean relative standard deviation was 0.28.4%. This analytical method could be successfully applied to the determination of 16 kinds of pesticides in drain-water samples from a golf course.