Abstract
The studies described in this paper are concerned with the effect of loading Kupffer cells on the destruction of DAB by rat liver slices. India ink, trypan blue, congo red, and carmine were used as agents to ′block′ the Kupffer cells.
DAB-destroying ability by rat liver slices were generally depressed by loading of various kinds of dyes. However, the degree of depression varied according to the kinds of dyes or the intervals after the time of loading. The depression of DAB-destroying ability in congo red group was most remarkable and with least fluctuation. Histologically, Kupffer cells in the liver loaded with those dyes revealed various degrees of hypertrophy in each group and stage of loading. The DAB-destroying ability of rat liver slices showed different values according to the degree of loading in the Kupffer cells. The hepatic parenchymal cells revealed no marked change during 48 hours after injection.
From these results it is presumed that Kupffer cells may play an important role in the destruction of DAB in the rat liver.
