Abstract
An attempt was made to use cultured Yoshida sarcoma cells as a meterial for metabolic and morphological studies of tumor cells, and some preliminary experiments were carried out to provide a basis for it.
Yoshida sarcoma cells collected after 24 hours' cultivation in a mixture of equal amounts of bovine serum and Earle's balanced saline (S-E) were used for these studies. As the incubation medium for the measurement of glycolysis and respiration, a mixture of equal amounts of bovine serum and either Krebs-Ringer bicarbonate (S-K) or Krebs-Ringer Tris-buffer was used.
1) It was confirmed that Yoshida sarcoma cells could survive and continue to multiply in the medium S-K (in which the glycolytic activity was measured) for at least 48 hours.
2) Cultured Yoshida sarcoma cells retained their glycolytic and respiratory activities for more than 3 hours in such conditions. A considerably high rate of 32P-incorporation into RNA-P or DNA-P by these cells was also observed during glycolysis and respiration.
3) The appearance of cultured Yoshida sarcoma cells remained normal and their mitotic figures were also found after the measurement of glycolysis and respiration.
4) The viability of cultured Yoshida sarcoma cells was proved by transplantation.
5) The effect of some agents was examined under the same conditions. In general, the degree of inhibition of 32P-incorporation into RNA-P or DNA-P by the cells was greater during glycolysis than during respiration.
6) From these results, it seems that cultured Yoshida sarcoma cells are advantageous as a material for such studies.
