A COMPARATIVE STUDY ON THE SPECIFIC GRAVITY OF MOUSE AND RAT LIVER CELL AND THEIR TUMOR CELL

Abstract

A comparative study on the cellular specific gravity of the regenerating rat liver, India ink-injected mouse liver, mouse liver tumor, liver of a mouse fed 2, 7-(diacetylamino)fluorene (2, 7-FAA), and transplantable rat liver cell carcinoma was performed by the method of multistrata-centrifuge.
The specific gravity of normal rat liver cell showed a range between 1085 and 1090, and that of mouse liver cell was between 1090 and 1095. The specific gravlty decreased in the regenerating rat liver cell, but became similar to that of the normal liver cell on the 7th day.
Third day after injection of India ink into mouse peritoneal cavity, liver cell was divided into three fractions by the specific gravity.
In the case of mouse liver tumor cell induced by 2, 7-FAA, the specific gravity showed lower value than that of the normal cell. Cells derived from non-tumorous part of tumor-bearing liver also had lower specific gravity.
Mouse liver during three stages of feeding 2, 7-FAA showed various values in each stage.
There were different populations in each generation of transplantable rat liver cell carcinomas, DEN-10893 (893) and DEN-10844 (844), 4) but all of them showed decreased value of the specific gravity compared with that of originating organ cell.
The meanings of the specific gravity of the cell were discussed from these results.