ACTIVITIES OF DNA POLYMERASE IN HUMAN UROGENITAL TUMORS

Abstract

The determination of DNA polymerase activities was made in various human urogenital tumors. In all the tissue examined, the enzyme showed higher activity in the presence of heat-denatured DNA than native DNA as the template. In some tumors which exhibited elevated activity of DNA polymerase, there was a large difference between the activity with native DNA and heat-denatured DNA. Few per cent of enzyme activity of whole homogenate remained in the nuclear fraction after the fraction was washed with isotonic sucrose solution. Among human urogenital tumors, relatively low activity of DNA polymerase was observed in benign prostatic hypertrophy and prostatic carcinoma, while relatively high activity was detected in testicular tumors, pelvic tumors of kidney, some cases of bladder tumor, and Grawitz tumor.