Abstract
A new cell line derived from newborn Chinese hamster lung tissue was established in in vitro culture. This cell line (referred to as CHL) grew as monolayers with a doubling time of about 30 hours, showing fibroblastic appearances in morphology. Chromosome analysis of CHL line revealed that 54% of metaphases were normal female diploid. Inoculation of the cells at low densities less than 200 cells per dish gave rise to discrete colonies different in size and feature, with a plating efficiency of 16-20%.
