ISOLATION OF NUCLEI FROM ASCITES HEPATOMA AH-130 CELLS OF RATS BY A DETERGENT

Abstract

The nuclei of AH-130 hepatoma cells were isolated with a nonionic surface-active agent. Nonidet P-40. The isolated nuclei were enclosed by only inner membrane and showed 0.64 as the ratio of RNA/DNA. Nucleotide compositions of nuclear RNA were not much different from those of nuclei isolated without the surfactant.