EFFECT OF ANTI-4-AZOQUINOLINE 1-OXIDE ANTIBODY ON IMMUNOSUPPRESSION BY 4-HYDROXYAMINOQUINOLINE 1-OXIDE

Abstract

Both humoral and cell-mediated immunity were suppressed by four intravenous injections of 100μg each of 4-hydroxyaminoquinoline 1-oxide, employing bacterial α-amylase and picryl chloride as antigens. Preimmunization of mice with diazotized 4-aminoquinoline 1-oxide-conjugated rabbit serum protein by the use of complete Freund's adjuvant prevented immunosuppression by 4-hydroxyaminoquinoline 1-oxide. Anti-hapten titers of these mice were 2⁵ to 2⁸ estimated by passive hemagglutination during the course of the experiments. Passive immunization by transfer of the specific anti-hapten antibody induced prevention from immunosuppression by the reagent. Therefore, it was suggested that the humoral anti-hapten antibody might play an important role in preventing mice from immunosuppression by the reagent by preimmunization with the hapten-carrier conjugate.