COVALENT BINDING OF 6-NITROBENZO-[a]PYRENE METABOLITES TO DNA IN VITRO

Abstract

Incubation of 6-nitrobenzo [a]pyrene[7, 10-¹⁴C] and calf thymus DNA with rat liver S9 fractions resulted in covalent binding of nitrobenzo[a]pyrene to DNA. In the presence of flavin mononucleotide under anaerobic conditions, the amount of binding decreased. Binding studies using synthetic poly-nucleotides showed that there was a high preference for poly(dG) compared to poly(dA), poly (dC) and poly(T).