REQUIREMENTS OF ADHERENT CELLS FOR ACTIVATING LYT-1⁺2^- T CELLS AS WELL AS FOR FUNCTIONING AS ANTITUMOR EFFECTORS ACTIVATED BY FACTOR(S) FROM LYT-1⁺2^- T CELLS

Abstract

The mechanism by which tumor-specific Lyt-1⁺2^- T cells exhibit their antitumor effect in collaboration with an adherent cell population was investigated with the use of a double diffusion chamber. The double diffusion chamber was prepared by separating the two chambers from each other by a Millipore membrane and implanted in the peritoneal cavity of C3H/He mice. When one chamber contained normal C3H/He spleen cells plus syngeneic viable MH134 hepatoma cells and the other contained normal C3H/He spleen cells plus syngeneic viable X5563 plasmacytoma cells, tumor cells in both chambers continued to proliferate. In contrast, the injection of spleen cells immunized to the MH134 tumor into one (the first) chamber containing MH134 tumor cells not only resulted in the growth inhibition of MH134 tumor cells, but also exhibited an appreciable inhibitory effect on the growth of X5563 tumor cells admixed with normal spleen cells in the other (second) chamber. The growth inhibition of X5563 tumor cells in the second chamber was mediated by Lyt-1⁺2^- T cells specific for MH134 tumor cells admixed in the first chamber. Such tumorspecific Lyt-1⁺2^- T cell function was dependent on the existence of adherent cells in the first chamber, and adherent cells in the second chamber were also required for the X5563 growth inhibition. In addition, when the second chamber containing adherent cells, instead of the connection to the first chamber, was provided with macrophage-activating factor (MAF), X5563 growth inhibition was also observed. These results indicate that adherent cells are required for activating tumor-specific Lyt-1⁺2^- T cells and for functioning as nonspecific antitumor effector cells activated by factor(s) such as MAF from Lyt-1⁺2^- T cells.