DETECTION OF AMPLIFIED DNA SEQUENCES IN GASTRIC CANCERS BY A DNA RENATURATION METHOD IN GEL

Hiroshi NAKATANI; Eiichi TAHARA; Teruhiko YOSHIDA; Hiromi SAKAMOTO; Toshimitsu SUZUKI; Hidenobu WATANABE; Morimasa SEKIGUCHI; Yasuhiko KANEKO; Masaharu SAKURAI; Masaaki TERADA; Takashi SUGIMURA

doi:10.20772/cancersci1985.77.9_849

Hiroshi NAKATANI, Eiichi TAHARA, Teruhiko YOSHIDA, Hiromi SAKAMOTO, Toshimitsu SUZUKI, Hidenobu WATANABE, Morimasa SEKIGUCHI, Yasuhiko KANEKO, Masaharu SAKURAI, Masaaki TERADA, Takashi SUGIMURA

Author information

Hiroshi NAKATANI
Genetics Division, National Cancer Center Research Institute
Department of Pathology, Hiroshima University, School of Medicine
Eiichi TAHARA
Department of Pathology, Hiroshima University, School of Medicine
Teruhiko YOSHIDA
Genetics Division, National Cancer Center Research Institute
Hiromi SAKAMOTO
Genetics Division, National Cancer Center Research Institute
Toshimitsu SUZUKI
Department of Pathology, Niigata University, School of Medicine
Hidenobu WATANABE
Department of Pathology, Niigata University, School of Medicine
Morimasa SEKIGUCHI
Department of Clinical Oncology, Institute of Medical Science, University of Tokyo
Yasuhiko KANEKO
Department of Cancer Chemotherapy, Saitama Cancer Center Hospital
Masaharu SAKURAI
Department of Cancer Chemotherapy, Saitama Cancer Center Hospital
Masaaki TERADA
Genetics Division, National Cancer Center Research Institute
Takashi SUGIMURA
Genetics Division, National Cancer Center Research Institute

Keywords: DNA renaturation method, Amplified DNA sequence, Gastric cancer, Gene amplification

JOURNAL FREE ACCESS

1986 Volume 77 Issue 9 Pages 849-853

DOI https://doi.org/10.20772/cancersci1985.77.9_849

Details

Abstract

A DNA renaturation method in gel was used to detect amplified DNA fragments in eight gastric cancer cell lines, KATO-III, OKAJIMA, SCH, MKNI, MKN7, MKN28, MKN45 and MKN74 cells, and tissues of three metastases of gastric cancers to lymph nodes. There were amplified DNA sequences in three gastric cancer cell lines. Judging from the intensities of the bands, HindIII-digested DNA fragments were amplified several hundred times in the KATO-III and MKN7 cell lines. DNA from OKAJIMA cell line contained multiple bands with less intensity. KATO-III cells were found to contain a homogeneously staining region in chromosome 11.

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