ESTABLISHMENT OF A PARATHYROID HORMONE-LIKE FACTOR-PRODUCING ESOPHAGEAL CARCINOMA CELL LINE (EC-GI)

Kanji SATO; Keizo KASONO; Yoshito OHBA; Toru YASHIRO; Yuko FUJII; Mitsuaki A. YOSHIDA; Toshio TSUSHIMA; Kazuo SHIZUME

doi:10.20772/cancersci1985.78.10_1044

Kanji SATO, Keizo KASONO, Yoshito OHBA, Toru YASHIRO, Yuko FUJII, Mitsuaki A. YOSHIDA, Toshio TSUSHIMA, Kazuo SHIZUME

Author information

Kanji SATO
Department of Medicine, Institute of Clinical Endocrinology, Tokyo Women's Medical College
Research Institute of the Foundation for Growth Science in Japan
Keizo KASONO
Department of Medicine, Institute of Clinical Endocrinology, Tokyo Women's Medical College
Yoshito OHBA
Department of Medicine, Institute of Clinical Endocrinology, Tokyo Women's Medical College
Toru YASHIRO
Department of Surgery, Institute of Clinical Endocrinology, Tokyo Women's Medical College
Yuko FUJII
Research Institute of the Foundation for Growth Science in Japan
Mitsuaki A. YOSHIDA
Department of Cytogenetics, Medical Research Institute, Tokyo Medical and Dental University
Toshio TSUSHIMA
Department of Medicine, Institute of Clinical Endocrinology, Tokyo Women's Medical College
Research Institute of the Foundation for Growth Science in Japan
Kazuo SHIZUME
Department of Medicine, Institute of Clinical Endocrinology, Tokyo Women's Medical College
Research Institute of the Foundation for Growth Science in Japan

Keywords: Esophageal carcinoma, Hypercalcemia, Parathyroid hormone, Bone resorbing factor

JOURNAL FREE ACCESS

1987 Volume 78 Issue 10 Pages 1044-1048

DOI https://doi.org/10.20772/cancersci1985.78.10_1044

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Abstract

A new cell line, designated EC-GI, was established from a 65-year-old patient with esophageal carcinoma who developed humoral hypercalcemia. The original tumor as well as the cell line caused marked hypercalcemia in tumor-bearing nude mice, in which a marked increase in osteoclastic bone resorption was demonstrated. The conditioned medium of EC-GI cells contained potent bone resorbing activity which stimulated cyclic AMP production in parathyroid hormone (PTH)-responsive osteoblast-like cells (ROS 17/2.8). EC-GI cells will be useful for characterization and purification of the PTH-like factor responsible for humoral hypercalcemia of malignancy.

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