Japanese Journal of Cancer Research GANN
Print ISSN : 0910-5050
Serological and Immunohistochemical Detection of a Gastrointestinal Cancer-associated Asialoglycoprotein Antigen of Human with a Murine Monoclonal Antibody
Masaaki ADACHITeruaki SEKINEKohzoh IMAIAkira YACHIShigeaki SATO
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1987 Volume 78 Issue 12 Pages 1370-1377


A murine monoclonal antibody (mAb), NCC-AS 13 (IgM, κ), was producedafter immunization with ascites from a gastric cancer patient. In a sandwich enzyme immunoassay, NCC-AS 13 reacted with the sera from 32% of 58 gastric cancer patients, 44% of 9 hepatocellular cancer patients and 33% of 6 colorectal cancer patients. Immunohistological analysis showed NCC-AS 13 to react with approximately 90% of 51 gastric carcinomas, 12 out of 12 colon carcinomas and 3 out of 4 lung carcinomas. The NCC-AS 13 defined antigen was determined to be a carbohydrate without terminal sialic acid on macromolecular glycoproteins. This mAb NCC-AS 13, detecting a novel antigen associated with gastrointestinal cancers, may have potential uses for tumor immunoscintigraphy and for the therapy of gastric cancer patients.

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© The Japanese Cancer Association
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