Japanese Journal of Cancer Research GANN
Print ISSN : 0910-5050
Antitumor Effect of Human Recombinant Interferon-γ Alone and in Combination with Adriamycin on a Human Colon Cancer Cell Line (RPMI 4788) in vitro and in Nude Mice
Hidenori KONDONoriaki TANAKAKunzo ORITA
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1987 Volume 78 Issue 12 Pages 1400-1408


The antitumor effect of recombinant human interferon-γ (rIFN-γ) and adriamycin (ADM), alone or in combination, against a human colon cancer cell line (RPMI 4788) was examined in vitro and in nude mice. In the in vitro study, antiproliferative activities of ADM were augmented additively or synergistically by combination with rIFN-γ in a dose-dependent manner. Growth of RPMI 4788 cells (2×106/mouse) transplanted subcutaneously into CD-1 nu/nu nude mice (day 0) was not inhibited significantly by 28 daily injections from day 10 of rIFN-γ alone, but the antitumor effect of ADM was augmented synergistically by the simultaneous injection of rIFN-γ. In an experimental model of pulmonary metastasis, in which RPMI 4788 cells (2×106/mouse) were inoculated intravenously into BALB/c nu/nu nude mice (day 0), 10 or 21 daily injections from day 2 of rIFN-γ alone had significant inhibitory effects against pulmonary metastasis in a dose-dependent manner. Furthermore, the inhibitory effect of ADM was augmented synergistically by daily injections of rIFN-γ.

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