Activation of N-ras Gene in a Rat Hepatocellular Carcinoma Induced by Dibutylnitrosamine and Butylated Hydroxytoluene

Tadao FUNATO; Jun YOKOTA; Hiromi SAKAMOTO; Toru KAMEYA; Shoji FUKUSHIMA; Nobuyuki ITO; Masaaki TERADA; Takashi SUGIMURA

doi:10.20772/cancersci1985.78.7_689

Activation of N-ras Gene in a Rat Hepatocellular Carcinoma Induced by Dibutylnitrosamine and Butylated Hydroxytoluene

Tadao FUNATO, Jun YOKOTA, Hiromi SAKAMOTO, Toru KAMEYA, Shoji FUKUSHIMA, Nobuyuki ITO, Masaaki TERADA, Takashi SUGIMURA

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Keywords: Rat hepatocellular carcinoma, Dibutylnitrosamine, Butylated hydroxytoluene, Butylated hydroxyanisole, N-ras

JOURNAL FREE ACCESS

1987 Volume 78 Issue 7 Pages 689-694

DOI https://doi.org/10.20772/cancersci1985.78.7_689

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Abstract

DNA samples from eighteen rat hepatocellular carcinomas, including those induced by oral administration of dibutylnitrosamine (DBN) with butylated hydroxytoluene (BHT), or DBN with butylated hydroxyanisole (BHA), have been tested for the presence of transforming activity by transfection assay with NIH3T3 cells. Of the eighteen samples, only one from a tumor induced by DBN and BHT gave transformants. The activated oncogene was identified as rat N-ras by Southern blot analysis.

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