Web-based BEST-KIT:Development of Web-based Biochemical Engineering System Analyzing Tool-KIT

Abstract

We have implemented an efficient, user-friendly, and web-based "biosimulator" named called BEST-KIT (Biochemical Engineering System analyzing Tool-KIT: http://helios.brs.Kyushu -u.ac.jp/˜bestkit/) for analyzing a a large scalelarge scale nonlinear reaction networks such as metabolic pathways. The BEST-KIT consists mainly consists of two kinds of modules, "MassAction" and "EnzymeKinetics.". In this study, we have developed a new module, "MassAction++, ", which can construct and analyze reaction schemes represented by both mass action law (mass balance) and approximated velocity functions of enzyme kinetics at steady state. All modules in BEST-KIT are developed in Java applet style, and can be carried outrun on the "any" platform machine through the web browser. The "MassAction++" module was developed by in Java applet style. andIt can save data related on to the constructed reaction scheme and time course data onto the client disk space and can load it onto the web browser.