2009 Volume 5 Issue 1 Pages 62-72
Extracellular levels of dopamine (DA) and serotonin (5-HT) were assayed in striatum of freely moving mice using microdialysis and high performance liquid chromatography with electrochemical detection (HPLC-EC), following oral administration of methylenedioxypyrovalerone (MDPV). As well as these experiment, we performed the measurement of locomotor and behavior activities, and examined whether central nerve injury was caused by repetitive administration using an immunohistochemical method. Dialysates were assayed at 10 minute intervals until 2.5 hours after an oral administration of MDPV. DA extracellular content was 210% of control at 0-30minutes and 208% at 30-60 minutes. But 5-HT was not significantly influenced by MDPV administration. Increasing of the DA level was more moderate by MDPV than other amphetamine like stimulants; methamphetamine and MDMA. It was thought that the augmentation of locomotor and behavior activities increased, when dopamine content became more than 200% of the control level in striatum. The neurotoxicity of MDPV was not observed in this experiment.
