Abstract
1) Experimental cholecystitis in dogs was produced by injecting E. coil (NIHJ and C-43 strain) and 5% sodium desoxycholate into the gallbladder. In postmortem examination, bile usually contained about 106/m1 of E. coil possessing same susceptibility patterns to the original microorganisms. Pathological studies of these. gallbladders revealed signs of inflammation.
2) The produced cholecystitis was treated with th ree antibiotics?chloramphenicol (CP), kanamycin (KM), aminobenzyl-penicillin (AB-PC)--for 1 week. More or less effects were obtained from the treatment with these drugs with the exception of KM. Especially, AB-PC group was most successfully treated. The cholecystitis produced with E. coil susceptible to CP responded more effectively than those with the microorganism moderately resistant to this antibiotic. In addition to these results, the observation of the blood and biliary concentration of these three antibiotics (100 mg/kg 1. m. ) for 24 hours, revealed the fact that the latter played more important role in the treatment of experimen tal cholecystitis.
3) Injecting E. coli adapted to 2% desoxycholate, in vitro, more severe inflammation was produced than the cases with unadapted microorganism.
4) Determination of hemaggl utinin titer was carried out in order to study the process of inflammation. In normal dogs without infection, average H. A. titer of 22 has obtained. In non-treated group without chemotherapy, significant high titer up to 28 was observed on 7 th day. In the treated group, fluctuation of the titer was observed in proportion to the effects of treatment. The grade and process of inflammation may be evaluated by the method of hemagglutination reaction.
