STUDIES ON CONCENTRATION OF BLEOMYCIN IN BLOOD, URINE AND TISSUES
1969 Volume 17 Issue 6 Pages 933-937
Details
1969 Volume 17 Issue 6 Pages 933-937
1) Concentration of bleomycin in the blood, urine, and tissues from cancer patients or experimental animals was estimated by means of bioassay method (thin plate-cup method using Bacillus subtilis, ATCC 6633 spores as the test organisms).
2) The maximum blood leve l was 3. 3 mcg/ml in the patients received intravenously 15 mg of the drug, and was 0. 97 mcg/ml in those received intramuscularly.
3) Relative high level of bleomycin was det ected in the kidney, lung, liver, skin and spleen in animal experiments (mice).
4) The drug le vel in the tumor was higher than that in the adjacent normal tissues of cancer patients. In tumor bearing mice, relative high or moderate level was obtained in the ascitic fluid and the solid tumor tissues, but very low in the ascitic tumor cells.
5) The drug was recovered at the ratio of 38. 4 % wit hin 24 hours in the urine of the patients received intravenously 15 mg of the drug.
6) The effect of inactivation or adsorption of the drug by various tissue homogenates from mice was very slight as comparing with other anti-cancer drugs.
