1970 Volume 18 Issue 2 Pages 157-166
1) The ability of E. coli (NIHJ) to grow in bile salt containing media was studied. E. coli was adapted by serial transfer in cholate and deoxycholate containing media to grow more rapidly than unadapted cultures.
2) No changes of biochemical nature of E. coli adapted by 7 times transfer in 1% cholate and 1% deoxycholate were shown comparing with unadapted E. coli.
3) In study of antibiotic susceptibility of unadapted, adapted and reversed E. coli, these 3 microorganisms showed the same values of minimal inhibitory concentration to cephaloridine (CER), kanamycin (KM), ampicillin (AB-PC) and chloramphenicol (CP).
4) Antibiotic susceptibility to CER, cephaloglycin (CEG), KM, deoxykanamycin, CP and AB-PC was studied in 25 strains of E. coli isolated from the patients with urinary tract infection and 14 strain, isolated from those with biliary tract infection. The patterns of susceptibility between these 2 groups of E. coli were different except CEG and CP. These differences were statistically significant (P<0.01-0.05).
5) Between adapted and unadapted E. coli, a marked difference in viable numbers in media with several antibiotics was demonstrated. The adapted E. coli grown in media containing CER, AB-PC or KM decreased more rapidly after 5 hours than the unadapted E. coli.
6) In 5 hours' culture in media containing CER and AB-PC, the decrease in viable counts of E. coli isolated from feces of the patients with the urinary tract infection was often found to be more significant than E. coli in urine from the same patients.
