STUDY OF STABILITY OF STREPTOMYCIN SULFATE BY BIOLOGICAL ASSAY METHOD
1975 Volume 23 Issue 8 Pages 2564-2568
Details
1975 Volume 23 Issue 8 Pages 2564-2568
Kinetics on degradation of streptomycin sulfate (SM) were investigated employing agar-plate disc assay method with Bacillus subtilis.
The minimum inhibitory concentration of SM was 10 pg/ml in this assay system without reference to pH of SM solution.
While, decrease in its activity was resulted in the loss of inhibiting zone around the discs, when SM solution adjusted to pH values ranging from 1. 5 to 9. 2 was heated at varying temperatures (60°C, 80°C and 90°C).
From both factors of time of heat-treatment and concentration of SM in disc for exhibiting the disappearance of inhibiting zone, stability constant (K) of SM was calculated according to the equation presented by us.
A series of experiments with SM solution pretreated under the stated conditions brought about the data to show that K values were found to be identical, having no relation with the concentration of SM in disc.
In addition, the results obtained by this biological assay method were almost similar to that obtained by a chemical analysis, azotometry.
