THE BIOASSAY METHOD OF PC-904 IN BODY FLUIDS

Abstract

Studies on the bioassay method of PC-904 concentration in body fluids were performed and the following results using Micrococcus luteus ATCC 9341 as an assay organism in heart infusion agar (p H 7.2) were obtained.
1) Detectable sensitivity limit of this method for PC-904 was 0.1-0.2 μ g/ml by thin layer cup method or 0.2-0.39 μ g./ml by paper disc method.
2) Standard curve of PC-904 using M/15 phosphate buffer (p H 7.0) as diluent of PC-904 as slightly different from that of fresh human serum or Moni-Trol I.