ON THE BINDING CAPACITY OF PCG POLYMERS TO SERUM PROTEIN

ESPECIALLY ON THE ANALYSIS BY CONTINUOUS FLOW COUNTERCURRENT DIALYSIS

Abstract

Benzylpenicillin polymers were isolated from stored PCG aqueous solution, and its binding capacity to BSA was comparatively studied by two dialyzing methods.
(1) By centriflow continuous dialyzer, free PCG was filtrated out completely within 30 minutes, showing no non-specific adsorption.
(2) By dialyzing method employing seamless cellulose tube, only 10% of free PCG was filtrated out for 30 minutes.
From these results, centriflow continuous dialyzing method was used for the test of protein binding capacity of PCG polymers. The result showed that 40% of PCG polymers (2mg) was bound to BSA (1mg), but the binding capacity did not change by increasing BSA.
These results suggested the existence of PCG polymers which did not bind with albumin.