Abstract
Studies on the assay procedure for the concentration of HCFU and its metabolites in organs were carried out using Staphylococcus aureus 209 P as the test organism.
Concentrations of HCFU and its metabolites in organs can be determined essentially by the procedure described previously. With several organs, such as stomach, small intestine and liver, however, it had been difficult to determine 5-FU concentration below 0.4 μg/g organ microbiologically because of an inhibitory effect of some organ-specific factor (factors) on formation of inhibition zone by 5-FU on the lawn of indicator bacteria.
This undesirable effect was eliminated by reextracting the aqueous phase containing 5-FU, with a mixture of propanol and ether.
Thus, we could determine 5-FU concentration in those organs with a lower limit of 0.05μg/g organ.
