1982 Volume 30 Issue 5 Pages 509-513
Tobramycin has a narrow therapeutic range. For proper dosage adjustment, it is necessary to have a fast, sensitive, specific and accurate assay technique for measuring serum levels of tobramycin. We examined high-performance liquid chromatography and three enzyme immunoassay methods; EMIT, substrate-labelled fluorescent immunoassay (SLFIA) and MARKIT to measure the concentration of tobramycin in clinical serum samples.
HPLC and EIA yielded values which compared favorably to bioassay in clinical serum samples (r=0.91-0.95).
MARKIT has such an advantage that it doesn't need any particular equipments. In the view of sensitivity, specificity, simplicity, we recommend EMIT and SLFIA.
