MICROBIOLOGICAL ASSAY OF AMOXICILLIN AND CLAVULANIC ACID IN SPECIMENS OF BODY FLUIDS AFTER BRL25000 (CLAVULANIC ACID-AMOXICILLIN) ADMINISTRATION

Abstract

BRL25000 is a 2 to 1 formulation of β-lactam compounds, namely the broad spectrum penicillin amoxicillin (AMPC), and the β-lactamase inhibitor clavulanic acid (CVA) respectively. Microbiological assay methods suitable for the measurement of amoxicillin and clavulanic acid in clinical specimens of serum and urine are described.
AMPC concentrations were measured by standard agar-diffusion microbiological assay using Micrococcus luteus ATCC9341 as assay organism. The CVA concentrations in body fluids were assayed using a β-lactamase producing strain of Klebsiella pneumoniae ATCC29665 in the presence of subinhibitory concentrations of penicillin G.
Fresh human serum and 0.1 M citrate buffer were used as body fluid diluents and studies showed that the AMPC and CVA content in serum and urine were stable for 2 weeks at temperatures below -55°C.