1990 Volume 38 Issue 6 Pages 548-551
Amphotericin B was incorporated into lipid emulsion using the method of KIRSH R et al. Shortly 20mg of sodium deoxycholate were dissolved in 1.0 ml of dimethylacetamide. This solution was used to solubilize the amphotericin B at various concentrations, and then Intralipos 20%® was added.
The LD50 of amphotericin B lipoid emulsion was more than 5.0 mg/kg in normal BALB/C mice, compared with 1.2 mg/kg for conventional amphotericin B (Fungizoneu®).
In a study of experimental murine disseminated candidiasis, the survival rate of mice given conventional amphotericin B at a dose of 8.0 mg/kg (the maximal tolerated dose without acute lethality) was 20%, whereas the animals treated with amphotericin B in lipid emulsion all were alive 42 days after inoculation (p<0.01).
In the case of experimental murine cryptococcosis, amphotericin B in emulsion also produced higher survival rates than commercial amphotericin B.
