In vitro activity of biapenem, a new carbapenem antibiotic
1994 Volume 42 Issue Supplement4 Pages 20-25
Details
1994 Volume 42 Issue Supplement4 Pages 20-25
The in vitro activity of biapenem (BIPM), a new carbapenem, was compared with those of imipenem (IPM) and ceftazidime (CAZ). BIPM was two-to fourfold less active than IPM against methicillin-susceptible Staphylococcus aureus at MIC90 of 0.1μg/ml. However, the activity of this compound exhibited low activities against methicillin-resistant S. aureus. The MIC90 of this compound for members of the family Enterobacteriaceae tested, including Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Proteus vulgaris, and Morganella morganii, ranged from 0.1 to 3.13μg/ml.BIPM also inhibited Pseudomonas aeruginosa at an MIC90 of 3.13μg/ml; it thus was twofold more active than IPM.
Morphological changes of cells of E.coli and P. aeruginosa following exposure to BIPM were observed under a phase-contrast microscopy from bacilli to large beans form and lysed easily.
Killing kinetics of this antibiotic for E.coli and P.aeruginosa showed that bactericidal action occurred at concentrations above the MIC, 0.05 and 0.39μg/ml, respectively. BIPM had a high affinity for PBP-2, -4, and lBs of E. coil and P. aeruginosa and PBP-1 and-4 of S. aureus.
