1994 Volume 42 Issue Supplement4 Pages 229-242
We established microbiological assay (Bioassay) and HPLC methods for biapenem (BIPM) in human body fluids and tissues, and studied on the stability of BIPM in human body fluid samples. The Bioassay methods established for BIPM in human body fluids are agar well and cup plate methods using Staphylococcus aureus Terajima as the test organism and Antibiotic medium 3 +1.5 % of Agar (pH 7.0) as the test medium, and paper disk method using Staphylococcus aureus IFO 14607 as the test organism and Nutrient agar +0.1% of N-Acetyl-D-glucosamine as the test medium. The quantitation limits of BIPM in agar well, cup and paper disk methods were 0.06, 0.04 and 0.06μg/ml, respectively. We also established reverse phase HPLC methods for BIPM in human body fluid samples. The quantitation limits of BIPM by the HPLC methods were 0.1 μg/ml in plasma and 1μg/ml in urine, respectively. There was a good correlation between the assay values by the Bioassay and the HPLC methods, and the correlation coefficients were 0.997 for plasma samples and 0.999 for urine samples, respectively. BIPM in the two-fold diluted solutions of human body fluids with 1M MOPS buffer or 50mm MOPS buffer·5% ethylene glycol mixture (1: 1) were stable for 4days at-20° and 53days at-80°C in plasma sample, and 7days at -20°C and 224days at -80°Cin urme sample, and 14days at -20°C and 28days at -80°C in bile samples, respectively.
