Development of a method that detect unique nucleic acid structure in cell

Abstract

It is becoming clear that RNA constructs G-quadruplex structures, and these structures regulate protein translation reaction in vitro and in cells. As a novel study of the field, researchers recently try to identify the “gene” that constructs RNA G-quadruplex structures(RGq) in mRNA and its “position.” Our group discovered a compound that has highly selectivity for RGq named RGB-1, and confirmed that it regulates translation reaction in cells. Using this advantage, we succeeded to discover a novel G-quadruplex formation site at the NRAS 5’UTR. Herein, we report that the method that detects RGq structures that regulate translation reaction in the cell using RGB-1.