Abstract
The protective agents for halophilic bacteria subjected to L-drying were screened by using Oceanospirillum multiglobuliferum IFO 13614 as an indicator and 75% seawater containing 5% monosodium glutamate, 1.5% adonitol and 1% sorbitol as a basal protective medium (BPM). Effects of 26 compounds on the survival of O. multiglobuliferum IFO 13614 were examined, and methly cellulose and L-proline were found as an effective agent. By the addition of 0.25% methyl cellulose 4000cps or 0.25% proline into BPM, the survival value of O. multiglobuliferum IFO 13614 increased to the value about 10-fold higher than that using BPM alone, and the protective effect was enhanced by using a mixture of these compounds. Furthermore, the mixture of methyl cellulose and proline was also effective for preservation of various halophilic bacteria.
