3. Cryopreservation of Cultured Plant Cells and Tissues at Super-Low Temperature II : Re-examination of Cryoprotectants(Papers presented at the 42nd Annual Meeting)

Abstract

Effects of glycerol, which is known as a poorly permeating cryoprotectant, on the cryopreservation of cultured plant cells of wheat (Triticum monococcum) were investigated. Survival of cells rapidly decreased when cryoprotective solutions containing glycerol or glycerol plus sucrose were added to the cells. However, an apparent cryoprotective effect of the combination of glycerol and sucrose was observed in the cells frozen to -30℃ or -196℃ although it was less effective than that of DMSO and glucose.