Cryobiology and Cryotechnology
Online ISSN : 2424-1555
Print ISSN : 1340-7902
Observation and Measurement of Frozen Biological Sample by 3-Dimensional Internal Structure Microscope
Hideo YOKOTAKen-ichi KUDOHToshiro HIGUCHIYasuyuki SAGARAGab-Soo Do
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JOURNAL FREE ACCESS

1998 Volume 44 Issue 1 Pages 1-9

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Abstract
Observation of internal structure of biological sample was carried out with newly developed 3-Dimensional Internal Structure Microscope (3D-ISM). A true colored 3-dimensional image of the sample with high resolution and high quality was obtained with this system compared with those image available with X-Ray CT, MRI or microtome used. The internal structure of a sample was obtained by observing each sliced side after each slicing, while the slicing and observing procedure was proceeded continuously. After repeatedly slicing a sample, the digital image data of the sectional views were transferred to a computer, where 3-dimensional image of the internal structure of the sample was reconstructed. This report describes the observation and the measurement of the biological sample with a developed device.
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© 1998 Japanese Society of Cryobiology and Cryotechnology
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