1. Preservation of Paraformaldehyde-fixed Human Platelets(the 44th Annual Meeting)

Abstract

To develop platelet substitutes, we prepared paraformaldehyde-fixed and lyophilized human platelets according to Read et al. Although rehydrated and lyophilized platelets (RL platelets) lost their ability of collagen-dependent aggregation, ristocetin-dependent aggregation remained 90% of intact platelets. Flow cytometric analysis demonstrated that membrane glycoprotein (GP) I b and GP III a were present on the surface of RL platelets. GMP-140, a marker of platelet acivation, was also detected. Our results showed that the RL platelets may substitute adhesion function, i.e., an important function of platelets to stop bleeding at injured vessel wall, but slight activation occurred during preparing them. We suggest that RL platelets may be hopeful as a platelet substitute.