Abstract
We examined the effect of post-thaw culture medium on the viability of cryopreserved plant cells to obtain the higher survival rate of the preserved cells. Addition of activated charcoal to the culture medium increased the survival rate of cryopreserved Lavandula vera cells. Elimination of ammonium ion from the culture medium also enhanced the survival rate of cryopreserved Lavandula and rice cells. Amino acids differed in their effect on the survival rate of cryopreserved Lavandula cells. These results indicate that post-thaw culture condition is an important factor to determine the survival rate of cryopreserved plant cells.