Cryobiology and Cryotechnology
Online ISSN : 2424-1555
Print ISSN : 1340-7902
9. Effect of Elements in Post-Thaw Culture Medium on the Survival Rate of Cryopreserved Plant Cells(the 46th Annual Meeting)
Akira KURIYAMAMasao YAMANAKatsumi WATANABE
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JOURNAL FREE ACCESS

2000 Volume 46 Issue 2 Pages 115-118

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Abstract
We examined the effect of post-thaw culture medium on the viability of cryopreserved plant cells to obtain the higher survival rate of the preserved cells. Addition of activated charcoal to the culture medium increased the survival rate of cryopreserved Lavandula vera cells. Elimination of ammonium ion from the culture medium also enhanced the survival rate of cryopreserved Lavandula and rice cells. Amino acids differed in their effect on the survival rate of cryopreserved Lavandula cells. These results indicate that post-thaw culture condition is an important factor to determine the survival rate of cryopreserved plant cells.
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© 2000 Japanese Society of Cryobiology and Cryotechnology
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