Cryobiology and Cryotechnology
Online ISSN : 2424-1555
Print ISSN : 1340-7902
14. Super-low Temperature Preservation of Two Species of Lavandula Cells (L. hybrid and L. stoechas)(the 47th Annual Meeting)
Akira KURIYAMAMasao YAMANAKatsumi WATANABE
Author information
JOURNAL FREE ACCESS

2001 Volume 47 Issue 2 Pages 127-130

Details
Abstract
Primary cultures of Lavandula hybrid and Lavandula stoechas were carried out. Adventitious shoots and callus with a high growth rate were obtained from the tissues of both species cultured on MS medium supplemented with appropriate concentrations of cytokinin and auxin. Subcultured callus cells of both species were cryopreserved in liquid nitrogen. Although cryopreserved cells died during the post-thaw culture on MS medium, recovery cultures were obtained on the medium from which ammonium ion was eliminated. All of the cells preserved by the vitrification method failed to recover from the frozen-thaw injury and died during the post-thaw culture.
Content from these authors
© 2001 Japanese Society of Cryobiology and Cryotechnology
Previous article Next article
feedback
Top