Abstract
During this work, We defined that vigorous growth on cryopreserved asparagus (Asparagus officinalis L.) regenerates were induced not by cryoprotectant, but by frozen-thawed treatment with different cryoprotectant 0.6M raffinose. And lateral buds of asparagus nodal segments freezing treated were dyed more deeply and extensively by 0.1% (w/v) 2, 3, 5-Triphenyl tetrazolium chloride (TTC) reduction pigment as time goes by. Histological examination showed that mitochondria were increasing after cryopreservation in the treated cell. Enhansed growth were observed by horseradish with slow freezing method, which was supposed that vigorous growth by cryopreservation might exist generally.
