Abstract
Shoot apices of blue honeysuckle (Lonicera caerulea L. var. emphyllocalyx Nakai) in vitrcrcultures were successfully cryopreserved utilizing two methods of encapsulation-dehydration and vitrification. Encapsulation-dehydration, however, seems preferable to vitrification, since the highest survival rate of apices (93.3±3.3%) in encapsulation-dehydration was higher than that (50.0±5.8%) in vitrification. The highest survival was achieved when moisture content in beads was reduced to 23% by air-drying with silica gel for 4 hours in encapsulation-dehydration. In both cases, cold acclimation of cultures at 5℃ for 2 weeks was essential. However, in the present study it was shown that immersion of alginate-coated apices for 16 hours in loading solution supplemented with 0.5M glycerol and 0.1mM salicylic acid resulted in a high survival rate (93.3±6.7%) that can eliminate the cold acclimation process.
