Abstract
Cultured rice cells and moss protonemata were preserved in liquid nitrogen by desiccation method. Although rice cells cultured in MS medium containing 3% sucrose did not survive desiccation, the cells cultured in the medium containing higher concentrations (0.4M or 0.6M) of sucrose survived desiccation and preservation in liquid nitrogen. Viability of the cells after preservation in liquid nitrogen was enhanced when cultured in the medium without ammonium ion. This result accorded with the result of rice cells preserved in liquid nitrogen by pre-freezing method. Moss protonemata without pre-culture with the higher concentration of sucrose survived desiccation and preservation in liquid nitrogen. However, for the higher survival rate after preservation, it was needed that the speed of desiccation was decreased.
