Abstract
Vitrification provides a general solution to the problem of organ preservation, because it allows to avoid both the direct and indirect damaging effects of freezing. Generally, water in the cell crystallizes below a melting point, and ice crystals cause destruction in the cell. This is a serious problem on freeze preservation. Cryoprotectant is a material that enables cells to cryopreserve. Ethylene glycol ((HO)CH_2CH_2(OH), EG) aqueous solution is well known as cryoprotectant. Fahy et al. reported the benefit of substituting the weaker glass-former EG to the strong glass-former 1,2-propanediol on toxicity in their solution. Therefore, investigating the nature of a glass transition of EG aqueous solution is very important. Using Modulated Temperature Differential Scanning Calorimetry, MDSC, we investigated thermal relaxation around a glass transition temperature (T_g) on EG solution (20, 30, 40, 50, 60, 70, 80 mol%). The underlying heating rate was 0.5 ℃/min, modulation amplitude ± 0.5 ℃, modulated period 100, 60, 40 s.
