Involvement of Protein Synthesis during Preculture in Development of Desiccation Tolerance in Suspension-Cultured Cells of Marchantia polymorpha L.(Papers presented at the 52nd Annual Meeting)

Abstract

We have found that desiccation tolerance of Marchantia polymorpha cells is increased by preculture in a medium with 0.5 M sucrose. During this preculture, sucrose accumulated and expression patterns of proteins changed in the cells. Desiccation tolerance had not fully developed when protein synthesis was inhibited by 10 μM cycloheximide (CHX) during preculture with sucrose. Glass transition temperature (Tg) of the precultured cells was measured using differential scanning calorimetry (DSC). Compared with that of precultured samples, Tg of precultured samples with CHX shifted to a low temperature. The results suggested that newly expressed proteins during preculture are not only involved in the development of desiccation tolerance but also influence Tg in suspension-cultured cells of M polymorpha.