Abstract
Protoplasts were isolated from cultured cells of Pogonatum inflexum by treatment of enzyme solution containing 2%(w/v) Driselase, 0.6 M mannitol and 5 mM CaCl_2. Isolated protoplasts were re-suspended in different liquid medium and desiccated in a chamber maintaining 50% humidity. Protoplasts survived the desiccation and a subsequent cryopreservation in liquid nitrogen (desiccation-cryopreservation). Composition of suspension medium influenced the survival rate of desiccated protoplasts. Desiccated and desiccated-cryopre served protoplasts were also cultured and clarified to retain the cell division potential.
