Abstract
Developments of cryopreservation techniques on primary rat cortical cells were carried out to observe the effect of cooling rates. Primary rat cortical cells were dispersed in the cryopreserving medium including 5 vol% dimethylsulfoxide, and then they were frozen by various cooling rates ranged from -3K/min to -150K/min. The survival ratio was estimated qualitatively by using the phase-contrast microscope. The feasibility of the neural activity was verified by measuring its spontaneous action potential with a multi-electrode array system. Based on these characterizations, the lower cooling rate of the dispersed sample was more feasible for the cryopreservation of the primary rat cortical cells.
